Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography
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چکیده
منابع مشابه
Correspondence of high levels of beta-exotoxin I and the presence of cry1B in Bacillus thuringiensis.
Examination of 640 natural isolates of Bacillus thuringiensis showed that the 58 strains (9%) whose supernatants were toxic to Anthonomus grandis (Coleoptera: Curculionidae) produced between 10 and 175 micro g of beta-exotoxin I per ml. We also found that 55 (46%) of a sample of 118 strains whose culture supernatants were not toxic to A. grandis nevertheless produced between 2 and 5 micro g/ml....
متن کاملDetermination of exotoxin in Bacillus thuringiensis cells.
The presence of exotoxin in Bacillus thuringiensis was demonstrated and its quantity in the cells determined. The concentration of exotoxin in the producing microorganism is approximately half the concentration of ATP. Exotoxin is produced at such a rate that the cell excretes 1/5 to 1/4 of its exotoxin content into the medium per minute.
متن کاملAn ABC transporter from Bacillus thuringiensis is essential for beta-exotoxin I production.
beta-Exotoxin I is a nonspecific insecticidal metabolite secreted by some Bacillus thuringiensis strains. Several studies of B. thuringiensis strains that have lost the capacity to produce beta-exotoxin I have suggested that there is a strong correlation between high levels of beta-exotoxin I production and the ability to synthesize crystal proteins. In this study, we showed that a mutant strai...
متن کاملToxicity of Bacillus thuringiensis beta-exotoxin to three species of fruit flies (Diptera: Tephritidae).
The current study describes toxic effects of the Bacillus thuringiensis beta-exotoxin toward 3rd instars of 3 fruit fly species: Anastrepha ludens (Loew), A. obliqua (Macquart), and A. serpentina (Wiedemann). The beta-exotoxin was highly toxic to all 3 species tested, with LC50 values calculated as 0.641, 0.512, and 0.408 microgram/cm2 of filter paper used to expose the larvae, for A. ludens, A...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1990
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.172.6.3172-3179.1990